Integrated Michaelis-Menten equation in DynaFit. 3. Application to HIV protease BIOKIN TECHNICAL NOTE TN-2016-03

The DynaFit software package (http://www.biokin.com/dynafit/) contains a builtin (“hard-coded”) implementation of the integrated Michaelis-Menten equation formulated in terms of the Lambert omega function. This built-in regression model can be used conveniently to determine the substrate kinetic parameters either from a single progress curve or from a collection of enzymatic progress curves analyzed globally. An illustrative example includes previously published data on the substrate kinetics of HIV protease. It is shown that even a single reaction progress curve can be used to determine KM and kcat as long as two conditions are met simultaneously: (1) the initial substrate concentration must be higher than the KM; and (2) the enzyme reaction must be allowed to proceed to full completion.